Animal serum plays a variety of roles in animal cell culture

Different cells prefer different environments, and serum plays a variety of roles in animal cell culture

This is not only the difference in the medium, but also the spatial density of cell growth. Some cells grow better and grow better. This usually belongs to cells that grow more slowly. For example, endothelial cells. Some cells have fewer numbers and grow better in a better state of cells, such as macrophages and some tumor cells. In particular, macrophages grow very fast and wall stickers very quickly, so a small number of cells should be left behind when passing on from generation to generation, so that the cell state will be better. Macrophages prefer to grow in bulk, with space between the stacks. If they grow together, the cell morphology will basically be poor, and the aging will be more, which is not good for future experimental results. Therefore, when culturing cells, we should explore the spatial density that cells prefer to grow.

(1) Provide growth regulators required for cell survival and proliferation.

(2) There are no more or less nutrients in the basal medium.

(3) It contains some matrix components that can be used to paste parietal cells.

(4) Provide vitamins, lipids, metal ions and other carrier proteins.

(5) In some cases, neutralizing toxic substances protect cells from damage.

(6) Provide a good buffer system for the culture medium.

(7) Provide protease inhibitors to protect cells from the damage of proteases released by dead cells.

Contamination is the enemy of cell culture, and preventing and avoiding contamination is one of the keys to successfully culturing cells. The sense of crisis must be carried out throughout the experiment, otherwise it will not only waste time, but also waste manpower and material resources, and even cause irreparability

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