MAM-PF2

Swiss MAM-PF medium is derived from the EXPRES series CHO cell culture medium. It has clear components and is suitable for culturing a variety of cells, such as CHO and BHK cells, and is also suitable for the expression of recombinant proteins in suspension culture. CHO and BHK cells are two types of cells widely used in recombinant protein expression. MAM-PF series medium does not contain L-glutamine to avoid adverse effects caused by L-glutamine degradation and amine accumulation. MAM-PF medium can be added with a very small amount of phenol red or without it. Serum-free medium is higher than the medium that requires the addition of serum, and it facilitates the purification and subsequent processing of expression products.

Most serum-free media available on the market contain a variety of proteins and/or proteolytic products of unknown composition. Therefore, the completely defined culture medium has huge technical advantages over other serum-added culture media and serum-free culture media on the market. From a risk control perspective, animal protein-free media are also extremely popular, while also avoiding the impact of shortages and instability of animal-based raw materials.

MAM-PF1, MAM-PF2, MAM-PF7d and MAM-PF7e, MAM-PF medium does not contain animal proteins and peptides, and does not contain hydrolysates of unknown composition. Users need to add L-glutamine (0.6 to 8 mM) by themselves before use.

DMEM High Glouse

DMEM medium is a modified medium using BME, and its amino acid and vitamin content is four times that of BME culture. DMEM medium contains non-essential amino acids and specific essential trace elements, and the concentration of sodium bicarbonate is also increased.

The glucose content of standard formula DMEM medium is 1000 mg/L, and the glucose content of high-glucose DMEM medium is 4500 mg/L. DMEM was used to culture mouse embryonic cells in the early days.

Today, DMEM medium is widely used for serum-free culture of ordinary and transformed mouse cells and chicken cells.