MAM-PF2

Swiss MAM-PF medium is derived from expres series CHO cell culture media. Its composition is clear, suitable for culturing a variety of cells, such as CHO and BHK cells, and also suitable for the expression of recombinant proteins in suspension culture. CHO and BHK cells are two types of cells that are widely used in recombinant protein expression. THE MAM-PF series media does not contain L-glutamine to avoid adverse effects due to L-glutamine degradation and amine accumulation. MAM-PF medium can be added in very small amounts of phenol red or not added. Serum-free medium is superior to serum-free medium, which facilitates the purification and subsequent treatment of expression products.

Most commercially available serum-free media contain a variety of proteins with ambiguous composition and/or proteolysis products. Therefore, the media with completely clear composition has a huge technical advantage over other serum-added media on the market, as well as serum-free media. From a risk control perspective, animal protein-free media is also extremely popular, while also avoiding the effects of animal raw material shortages and instability.

MAM-PF1, MAM-PF2, MAM-PF7d and MAM-PF7e, MAM-PF medium contains no animal proteins and peptides, and no hydrolysis of unclear composition. Users need to add L-glutamine (0.6 to 8 mM) before use.

DMEM High Glouse

DMEM medium is a BME modified medium with four times the amino acid and vitamin content of BME culture. DMEM medium contains non-essential amino acids and specific essential trace elements, and the concentration of sodium bicarbonate is also increased.

The standard formulation DMEM medium has a glucose content of 1000 mg/L and a high-sugar DMEM medium of 4500 mg/L. DMEM was used in the early days to culture mouse embryonic cells.

TODAY DMEM media is widely used for serum-free culture of plain and transformed mouse and chicken cells