SF-4 Baculo Express ICM

Use directly without adding other ingredients

Introduction to Insect Cell Culture

The nutrients required by insect cells are generally similar to those of vertebrate cells, but there are still differences, and these differences should be noted when designing cell culture media.

steroids

Insects themselves cannot synthesize steroids, so the culture medium should provide synthetic precursors for insect cell membranes and ecdysone.

amino acids

The amino acid content in insect blood is very high, and the amino acid content in the culture medium should also be high.

organic acid

The content of free organic acids in insect blood is usually high, such as citric acid, succinic acid, oxalic acid and malic acid. The free organic acid content in each insect is 0.1-30 mmol.

pH, buffers and acid-base indicators

Insect tissue fluid is acidic, with a value of 6.2-6.9, so the pH value of insect cell culture medium should be between 6.2-6.5. The pH of most mammalian cell culture media is between 7.1-7.6. SF4 Baculo Express is able to maintain pH in different culture environments, such as exposure to air, or in closed containers. Insect cell culture media are buffered with sodium phosphate and do not require carbon dioxide to maintain pH. Insect cell culture medium does not require the addition of acid-base indicators. Insect cell culture media supplemented with protein hydrolysates therefore appear yellow.

Osmotic pressure

The osmotic pressure of insect cells is very different from that of vertebrates, almost twice that of vertebrates. Therefore, the osmotic pressure of insect cell culture medium is 340-390 mOsmol/kg, and that of vertebrate cell culture medium is 290-330 mOsmol/kg.

Glutamine and Glucose

Excessive metabolism of glutamine and glucose during mammalian cell culture results in excessive production of ammonium and lactate, and the accumulation of these metabolites is usually inhibited. The catabolite pathways of insect cells are different from those of mammalian cells, so higher levels of glutamine and glucose can be added to the insect cell culture medium to maintain high-density cell growth.

Serum-free culture using SF-4 Baculo Express medium

SF-4 Baculo Express medium uses a patented formula and has good performance in culturing different Spodoptera frugiperda cell lines (such as SF9, SF21), BTI-TN-5B1-4 cell line (High FiveTM) and Drosophila cell lines. .