SF-4 Baculo Express ICM

Use directly without adding additional ingredients

Introduction to insect cell culture

The nutrients required by insect cells are generally similar to those required by vertebrate cells, but there are still differences, which should be noted when designing cell culture media.

steroid

Insects themselves cannot synthesize steroids, and the medium should provide synthetic precursors for insect cell membranes and molting hormones.

amino acid

Insects have a high content of amino acids in their blood, and the amino acid content in the medium should also be high.

Organic acids

The amount of free organic acids in the blood of insects is usually higher, such as citric acid, succinic acid, oxalic acid and malic acid. The free organic acid content in each insect is 0.1-30 mmol.

pH, buffers, and acid-base indicators

Insect tissue fluid is acidic and has a value of 6.2-6.9, so the pH of insect cell culture medium should be between 6.2-6.5. Most mammalian cell culture media have pHs between 7.1 and 7.6. The SF4 Baculo Express is capable of maintaining pH in different culture environments, e.g. when exposed to air or in an airtight container. The buffer of insect cell culture medium is sodium phosphate and does not require carbon dioxide to maintain pH. Insect cell culture media does not require the addition of acid-base indicators. Therefore, insect cell culture medium with added protein hydrolysate has a yellow color.

osmotic pressure

The osmolality of insect cells differs greatly from that of vertebrates, almost twice that of vertebrates. Therefore, the osmolality of insect cell culture medium is 340-390 mOsmol/kg, and the vertebrate cell culture medium is 290-330 mOsmol/kg.

Glutamine and glucose

Excessive metabolism of glutamine and glucose during mammalian cell culture leads to the production of excess ammonium salts and lactates, and the accumulation of these metabolites is usually inhibited. Insect cell catabolite pathways are not as good as mammalian cells, so higher levels of glutamine and glucose can be added to insect cell culture medium to maintain high cell density growth.

Serum-free culture was performed using SF-4 Baculo Express medium

SF-4 Baculo Express medium uses a patented formula to culture different grass moth cell lines (e.g., SF9, SF21), BTI-TN-5B1-4 cell lines (High FiveTM) and Drosophila cell lines.